Determination of alosetron in human plasma or serum by high-performance liquid chromatography with robotic sample preparation |
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Authors: | Thomas L Lloyd Samir K Gupta Ann E Gooding John R Alianti |
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Institution: | Glaxo Research Institute, 5 Moore Drive, Research Triangle Park, NC 27709, USA |
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Abstract: | A method of analysis for the determination of alosetron in human plasma or serum has been developed. The method was fully automated using a laboratory robot in order to improve analytical precision, efficiency and safety. The assay involved solid-phase extraction with reversed-phase HPLC separation and fluorescence detection. A validation exercise over the concentration range of 0.1 to 20 ng/ml demonstrated the selectivity, linearity, sensitivity, accuracy, precision, extraction efficiency, ruggedness and stability of the method. The method has been applied in support of numerous human pharmacokinetic/biopharmaceutic studies over the last five years. |
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Keywords: | Alosetron Robotic sample preparation |
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