Sulphate transport inCandida utilis |
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Authors: | J A Benítez A Alonso J Delgado A Kotyk |
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Institution: | (1) Department of Molecular Genetics, Centro Nacional de Investigaciones Científicas, P.O. Box 6990, Havana, Cuba;(2) Department of Cell Physiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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Abstract: | Sulphate uptake byCandida utilis follows Michaelis-Menten type kinetics characterized by a Km of 1.43 mM for sulphate. The process is unidirectional, pH, temperature and energy dependent. Molybdate, selenate, thiosulphate,
chromate and sulphite are competitive inhibitors. Dithionite is a mixed-type inhibitor of sulphate uptake. If cells are pre-incubated
with sulphate, sulphite, thiosulphate, dithionite or sulphide, sulphate uptake is severely blocked. Inhibition by endogenous
sulphate, sulphite and thiosulphate was specific for sulphate uptake. Thus, incorporation of extracellular sulphate seems
to be under the control of a heterogeneous pool of sulphur compounds. These results are discussed in connection with the regulation
of sulphur ammo acid biosynthesis inC.utilis. |
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