Isolation of a copper complex and its rate of appearance in roots of Mimulus guttatus |
| |
Authors: | N. J. Robinson D. A. Thurman |
| |
Affiliation: | (1) Department of Botany, University of Liverpool, L69 3BX Liverpool, UK;(2) Present address: Genetics Group, Los Alamos National Laboratory, 87545 Los Alamos, NM, USA |
| |
Abstract: | A Cu-complex was isolated from the roots of copper-tolerant Mimulus guttatus. The elution volume of the complex determined by gel permeation chromatography was similar to that of rat-liver cadmium thionein. The complex was heat stable, had a relatively high ratio of absorbance at 254 nm: 280 nm and incorporated 35S. The complex, purified using a combination of gel permeation chromatography and anion-exchange chromatography, contained more glutamine/glutamic acid and glycine residues than mammalian metallothioneins. The amount of the complex in roots increased after 5 h growth in a solution containing 16 M Cu. Induction was preceded by an increase in the concentrations in root tissue of unknown compounds containing sulphur which may serve as precursors. The availability of these compounds appeared to regulate the rate of synthesis of this Cu-complex.Abbreviations CuBP copper-binding protein - HPLC highperformance liquid chromatography - MT metallothionein - Th thionein - Tris 2-amino-2-(hydroxymethyl)1-3-propanediol |
| |
Keywords: | Copper tolerance Metallothionein Mimulus (copper tolerant) |
本文献已被 SpringerLink 等数据库收录! |
|