Restriction enzyme BstZ17I is sensitive to cytosine methylation |
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| Authors: | Chandrasekhar Kanduri |
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| Affiliation: | 1. Cleveland Clinic, Genomic Medicine Institute, Cleveland, OH, United States;2. Kent State University, Departoment of Biomedicine, Kent, OH, United States;3. Cleveland Clinic, Lou Ruvo Center for Brain Health, Cleveland, OH, United States;4. Indiana University, Department of Neuroscience, Indianapolis, IN, United States;5. Cleveland Clinic, Center for Neurological Restoration, Cleveland, OH, United States |
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| Abstract: | The cleavage patterns of a subset of restriction enzymes are blocked or impaired when a methylated CpG is overlapped with either the 5' or 3' end of the canonical restriction site. BstZ17I restriction endonuclease is a blunt-end cutter, which recognises the hexanucleotide sequence GTA(downward arrow)TAC. In this report, I show that the BstZ17I restriction enzyme is sensitive to cytosine methylation. Using both in vitro-methylated episomal plasmids and lambdaDNA, I demonstrate that the BstZ17I restriction enzyme is sensitive to cytosine methylation that occurs 3' and/or 5' of the canonical recognition sequence. |
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| Keywords: | BstZ17I Cytosine methylation |
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