| 聚合酶链反应检测宫颈癌中HPV16E6／E7相关序列 |
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| 引用本文： | 刘学锋 杨平. 聚合酶链反应检测宫颈癌中HPV16E6／E7相关序列[J]. Virologica Sinica, 1992, 7(1): 59-62 |
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| 作者姓名： | 刘学锋 杨平 |
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| 作者单位： | 湖北医学院病毒所，湖北医学院病毒所，湖北医学院病毒所，湖北医学院病毒所，湖北医学院病毒所，湖北医学院病毒所 武汉 430071 湖北医学院生化教研室，武汉 430071，武汉 430071，武汉 430071，武汉 430071，武汉 430071 |
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| 摘 要： | 本文报告了采用聚合酶链反应(即PCR技术)检测人宫颈癌中人乳头瘤病毒16型(HPV16)的E_6/E_7相关序列。在正链引物(SP)和反链引物(ASP)的诱导下,以二步温控法聚合酶链反应对34例人宫颈癌组织DNA进行检测,发现宫颈癌中HPV16E_6/E_7相关序列存在的阳性率达67.6％(23/34)。本研究结果提示:HPV16可能在宫颈癌的发生发展过程中起重要作用,采用PCR技术选择性地检测HPV16的转化基因(E_6、E_7)对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。
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| 关 键 词： | 人乳头瘤病毒 宫颈癌 聚合酶链反应 |
Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E_6/E_7 in Cervical Cancers |
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| Liu Xue feng Virus Research Institute,Hubei Medical College,Wuhan. Use of Polymerase Chain Reaction for Detection of the Seguence Related to HPV16 E_6/E_7 in Cervical Cancers[J]. 中国病毒学(英文版), 1992, 7(1): 59-62 |
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| Authors: | Liu Xue feng Virus Research Institute Hubei Medical College Wuhan |
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| Affiliation: | Liu Xue feng Virus Research Institute,Hubei Medical College,Wuhan 430071 |
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| Abstract: | Detection of the sequence related to E_6/E_7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E_6/E_7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E_6/E_7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and use of the PCRtechnique for selective detecting the transforming genes(E_6/E_7) of HPV16 maybe helpful with research of cervical carcinogenesis and early finding of cervicalcancer patients. |
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| Keywords: | Human Papillomavirus Transforming genes Cervical cancers Polymerase chain reaction |
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