Efficient secretion of human endostatin in the yeast, Pichia pastoris |
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| Authors: | Jubao Duan Xin Cai Baolin Zhang Yuzhen Li Minji Zou Jiaxi Wang |
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| Institution: | (1) Laboratory of Molecular Genetics, Institute of Basic Medical Sciences, Beijing, 100850, P.R. China;(2) Laboratory of Microcirculation, The Great Wall Hospital, Beijing, 100853, P.R. China |
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| Abstract: | Endostatin is a 20 kDa COOH-terminal fragment of collagen XVIII that inhibits angiogenesis and tumor growth. The cDNA coding for human endostatin in human fetal liver has been cloned into the secreting expression organism Pichia pastoris, and the high level expression of human endostatin has been achieved (about 200 mg of endostatin in 1 l of culture). The recombinant human endostatin was purified to homogeneity by heparin-affinity column, and showed antiproliferative effect on rat brain micro-vascular endothelia cells. |
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| Keywords: | endostatin expression Pichia pastoris |
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