A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome |
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Authors: | Andreas Houben Carolyn R. Leach Dawn Verlin Ruth Rofe Jeremy N. Timmis |
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Affiliation: | (1) Department of Genetics, The University of Adelaide, SA 5005, Australia, AU |
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Abstract: | Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome. Received: 30 May 1997; in revised form: 20 August 1997 / Accepted: 20 August 1997 |
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