Characterization of clonal populations of theHeliothis zea cell line IPLB-HZ 1075 |
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Authors: | Bartholomew G Corsaro M J Fraser |
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Institution: | (1) Department of Biological Sciences, University of Notre Dame, 46556 Notre Dame, Indiana |
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Abstract: | Summary A total of 24 clones (HZ 1075/UND-A through X) were initially isolated by dilution plating from the established IPLB-HZ 1075
cell line. Many of the isolates with highly vacuolated cytoplasms eventually died during subculturing. The cloned cell strains
differed in their predominant morphology, cell doubling times, and relative ability to support replication of the singly encapsulated
nuclear polyhedrosis virus ofHeliothis zea (HzSNPV). The origin of the cloned cell strains was confirmed by comparing their isozyme profiles with those of the parental
IPLB-HZ 1075 cell line andH. zea larvae using stains for fumerate hydratase, lactate dehydrogenase (LDH), and malic dehydrogenase (MDH). One dipteran and
several lepidopteran cell lines maintained in our lab were also separable using stains for LDH and MDH.
This research was supported in part by USDA grant number GAM 8400211, and by a Jesse Jones Faculty Research Award from the
University of Notre Dame to M. J. F. |
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Keywords: | Lepidoptera Heliothis zea IPLB-HZ 1075 isozyme clones |
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