感染病毒的小麦全蚀菌的超微结构

将感染病毒的小麦全蚀菌山东烟台株培养 20天的菌体细胞,进行超微结构的研究。于电镜下观察到球状病毒颗粒,平均直径23—30nm,多是无规则松散的分布于胞质中;或紧密聚集于液泡、线粒体周围;或排列成线状;或7—8个颗粒排列成环状。病毒仅分布于细胞质中,细胞核、脂肪体内均未见病毒颗粒。病毒浓度在较老的菌体内有增加的趋势。全蚀菌的菌丝细胞壁有三层,外层电子致密内含纤维状物,内层电子较为透明,中层为一电子致密度很深的狭窄夹层。壁的厚度不均,外缘不规则;在菌丝体产生隔膜的早期阶段,于隔膜附近有1—3个外被膜结构的沃罗宁体 Woronin body,隔膜形成的后期,见电子致密物质沉积在核膜孔上,形成中的隔膜顶端为尖状突起向基部逐渐增宽略成金字塔形。

ULTRASTRUCTURE OF VIRUS INFECTED HYPHAL CELLS IN CAEUMANNOMYCES GRAMINIS

Ultrastructural studies on virus infected cells in Gaeumanomyces gra- minis collected on wheat plant from Yantai, Shandong Province, were carried out. Under electron microscope, spherical virus particles appeared in hyphal cells after 20 days in- cubation at 20℃. These particles were approximately 23-30 nm in diameter. They were scattered as individuals, unorganized loose aggregated or closely aggregated in cy- toplasm. In some cases, they were aggregated to form linear aggregates and associated with membraneous structures or 7-8 particles arranged around a hollow center and for- med circular structures. These virus particles were localized in the cytoplasm near the plasmalemma and could not be found in nuclei, lipid bodies or vacuoles. Large quan- tities of virus were observed in aged cells. Besides, ultrasections showed that the cells wall of Gaeumannomyces graminis had three layers: the outer electron dense layer, an inner electron transparent layer and a thin middle layer more opaqus than the other two. The thickness of the outer wall layer is 120-130 nm. At the early stage in the development of septa in a hypha, membrane bound Woronin bodies could be seen close to the septa, and septal pores might be plugged by electron dense material deposited along the tapered septa.