Study of peripheral blood lymphocyte subset distribution and IL-2 receptor (IL-2 R) p55–p75 subunit expression in patients with cancer of different sites |
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Authors: | Mantovani G Macciò A Lai P Turnu E Del Giacco G S |
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Institution: | (1) Department of Medical Oncology, University of Cagliari, 09124 Cagliari, Italy |
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Abstract: | The aim of the study was to evaluate the subset distribution and the IL-2 R p55–p75 subunit expression on unstimulated and
phytohemagglutinin (PHA)-stimulated (at 3-d) peripheral blood mononuclear cells (PBMC), of patients with solid cancers of
different sites. Indeed the expression of the two subunits of IL-2R is an essential prerequisite for The action of the IL-2
on CD8+, CD16+ lymphocytes as effectors in antitumor activity (LAK-cells). The subset distribution (CD3, CD4, CD8, CD16, DR) was assessed
by cytofluorometry with specific monoclonal antibodies (MAbs); the p55 (CD25) and p75 subunit expression was evaluated by
specific MAb (OKT26a and anti-p75). Ninety patients with advanced cancer (mainly non-small cell lung cancer NSCLC], head
and neck cancer, and gynecological cancer; mean age 55 yr; range 27–80) were studied. Thirty-five age- and sex-matched healthy
subjects were studied as controls. Our data show that there is no significant difference in the subset distribution between
cancer patients and controls. Furthermore, no difference has been found in the expression of p55 subunits on unstimulated
PBMC between cancer patients and controls. No difference has been found in the expression of both p55 and p75 subunits on
PHA-stimulated PBMC between cancer patients and controls. Our results can support the rationale for further clinical trials
with IL-2 in solid malignancies. |
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Keywords: | Cancer patients flow cytometry IL-2 lymphocyte subsets membrane-bound IL-2 R monoclonal antibodies peripheral blood mononuclear cells p55– p75 subunits |
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