Purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by Bordetella pertussis and Bordetella bronchiseptica |
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Authors: | Timothy J. Brickman Jan-Gerd Hansel Marvin J. Miller Sandra K. Armstrong |
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Affiliation: | (1) Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC, USA;(2) Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA;(3) Department of Microbiology and Immunology, East Carolina University School of Medicine, 27858 Greenville, NC, USA |
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Abstract: | Hydroxamate siderophores of virulent Bordetella pertussis and Bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(S),11,18(S)-tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone, which was previously isolated from the taxonomically-related bacterial species Alcaligenes denitrificans subsp. xylosoxydans. Alcaligin purified from iron-depleted cultures of B. pertussis and B. bronchiseptica exhibited specific growth-promoting activity under iron-restricted conditions for Bordetella indicator strains, and were active in [55Fe]ferric alcaligin transport assays. Evidence suggests that several C2-symmetric conformations of alcaligin exist simultaneously in both methanolic and aqueous solution. |
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Keywords: | iron siderophore alcaligin Bordetella pertussis Bordetella bronchiseptica siderophore purification iron transport |
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