Crystallization of DNA fragments from water-salt solutions,containing 2-methylpentane-2,4-diol |
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| Authors: | V. D. Osica B. Ya. Sukharevsky V. N. Vasilchenko B. I. Verkin O. F. Polyvtsev |
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| Affiliation: | (1) Physico-technical Institute of Low Temperatures, Academy of Sciences of the Ukr.S.S.R., Kharkov, USSR;(2) Institute of Biological Physics, Academy of Sciences of the U.S.S.R., Puschino-on-Oka, USSR |
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| Abstract: | Fragments of calf thymus DNA have been crystallized by precipitation from water-salt solutions, containing 2-methylpentane-2,4-diol (MPD). DNA crystals usually take the form either of spherulites up to 100  in diameter or of needles with the length up to 50 . No irreversible denaturation of DNA occurs during the crystallization process. X-ray diffraction from dense slurries of DNA crystals yields crystalline powder patterns. |
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