Penicillin binding proteins of Vibrio cholerae |
| |
| Authors: | T K Sengupta A N Chatterjee J Das |
| |
| Institution: | Biophysics Division, Indian Institute of Chemical Biology, Calcutta. |
| |
| Abstract: | Eleven penicillin binding proteins (PBPs) of Vibrio cholerae have been identified using 125I] labelled p-hydroxybenzyl penicillin (PenX). These proteins are localised in the inner membrane and have molecular weights ranging from 97,000 to 22,000. Neutral hydroxylamine released the labelled PenX from the PBPs and pretreatment with cold benzyl penicillin inhibited labelling completely. The PBP 4 is the most sensitive target for cephaloridine and aztreonam. Cephaloridine also binds to three other high molecular weight PBPs, 1, 2 and 3. Aztreonam, in addition to PBP 4, has affinity for another low molecular weight PBP, PBP 7. Mecillinam has affinity for PBPs 1, 4 and 11. |
| |
| Keywords: | |
|
|
