Structure of a major glycolipid from Thermus oshimai NTU-063 |
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Authors: | Lu Tzu-Li Chen Chien-Sheng Yang Feng-Ling Fung Jim-Min Chen Mao-Yen Tsay San-San Li Jianjun Zou Wei Wu Shih-Hsiung |
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Affiliation: | Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan. |
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Abstract: | The structure of a major glycolipid isolated from the thermophilic bacteria Thermus oshimai NTU-063 was elucidated. The sugar and fatty acid compositions were determined by GC-MS and HPLC analysis on their methanolysis and methylation derivatives, respectively. After removal of both O- and N-acyl groups by alkaline treatment, the glycolipid was converted to a fully acetylated tetraglycosyl glycerol derivative, the structure of which was then determined by NMR spectroscopy (TOCSY, HSQC, HMBC). Thus, the complete structure of the major glycolipid from T. oshimai NTU-063 was established as beta-Glcp-(1-->6)-beta-Glcp-(1-->6)-beta-GlcpNAcyl-(1-->2)-alpha-Glcp-(1-->1)-glycerol diester. The N-acyl groups on the 2-amino-2-deoxy-glucopyranose residue are C15:0 and C17:0 fatty acids, whereas the fatty acids of glycerol diester are more heterogeneous including both straight and branched fatty acids from C15:0 to C18:0. |
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Keywords: | Thermus oshimai Thermophilic bacteria Polar glycolipid NMR Mass spectroscopy |
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