Binding of 5S estradiol receptor to poly-deoxynucleotides |
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Authors: | M Sluyser S G Evers T Nijssen |
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Affiliation: | Department of Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands. |
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Abstract: | Calf uterus cytosol was incubated with (3H)estradiol and fractionated on Sephadex G-200. Two (3H)estradiol-binding protein fractions were obtained with sedimentation coefficients of 5.1 S and 3.5 S, respectively. The 5.1 S fraction bound to poly dT, poly dA:dT and poly dG:dC to a higher extent than to calf thymus DNA. The 3.5 S fraction did not bind to DNA. |
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