| AtAAPT1基因RNAi的植物表达载体的构建 |
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| 引用本文： | 郑桂灵,李鹏. AtAAPT1基因RNAi的植物表达载体的构建[J]. 植物研究, 2011, 0(3): 313-317 |
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| 作者姓名： | 郑桂灵 李鹏 |
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| 作者单位： | 西南科技大学生命科学与工程学院; |
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| 基金项目： | 西南科技大学博士基金“利用RNAi技术对植物膜脂PC功能的研究”(08zx7106) |
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| 摘 要： | 以拟南芥中氨基乙醇磷酸转移酶基因AAPT1作为RNA i的靶向序列,采用RT-PCR的方法获得目的DNA片段。然后以pBS-T-AAPT1载体为基础,构建了由35 s启动子调控的AAPT1基因RNA i的植物表达载体pART27-AAPT1(1,2),并通过电击法将重组质粒导入根癌农杆菌C58中。AtAAPT1基因RNA i的植物表达载体的构建对于研究AAPT1基因的功能和应用具有重要的理论价值。
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| 关 键 词： | AAPT1基因 RNAi 植物表达载体 |
Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi |
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| ZHENG Gui-Ling LI Peng. Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi[J]. Bulletin of Botanical Research, 2011, 0(3): 313-317 |
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| Authors: | ZHENG Gui-Ling LI Peng |
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| Affiliation: | ZHENG Gui-Ling LI Peng(School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621000) |
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| Abstract: | Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper.The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction.A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T,including AAPT1 gene controlled by CaMV35s promoter in this experiment.Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way. |
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| Keywords: | AAPT1 gene RNAi plant transformation vector |
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