Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
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Authors: | MR Scrochi CN Zanuzzi N Fuentealba F Nishida ME Bravi ME Pacheco |
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Institution: | 1. Departments of Virology;2. Histology and Embryology;3. National Scientific and Technical Research Council (CONICET), Argentina;4. Histology and Embryology;5. Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata, La Plata;6. Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata, La Plata;7. Department of Analytical Chemistry, School of Exact Sciences, National University of La Plata, La Plata |
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Abstract: | Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle. |
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Keywords: | apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells |
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