Engineering recombinant antibodies for immunotherapy |
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Authors: | Dario Neri Heike Petrul Gabrio Roncucci |
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Institution: | (1) Cambridge Centre for Protein Engineering, MRC Centre, Hills Road, CB2 2QH Cambridge, UK;(2) Molteni Farmaceutici, Casella Postale 2268, 50100 Firenze, Italy |
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Abstract: | Recombinant antibody fragments binding with high affinity to their target can be obtained either from hybridomas or directly
from antibody libraries on filamentous phage. These fragments are devoid of any activity other than antigen binding, and have
to be processed and functionalized in order to be suitable for clinical applications. This article presents the authors’ view
on the procedures and the features that are important for effective transformation of recombinant antibodies into useful immunotherapeutic
agents. The topics presented include phage display methodologies, engineering of high-affinity binding, purification, and
functionalization strategies of recombinant antibodies. |
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Keywords: | Antibody engineering immunotherapy recombinant antibodies |
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