| 工业微生物中NADH的代谢调控 |
| |
| 引用本文： | 秦义,董志姚,刘立明,陈坚. 工业微生物中NADH的代谢调控[J]. 生物工程学报, 2009, 25(2): 0161-0169 |
| |
| 作者姓名： | 秦义 董志姚 刘立明 陈坚 |
| |
| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214122 |
| |
| 基金项目： | 国家杰出青年基金(No. 20625619), 国家自然科学基金(Nos. 30670066, 20706025), 国家“863计划”(No. 2006AA02Z201)和国家“973项目” (No. 2007CB7l4303)资助。 |
| |
| 摘 要： | NADH是微生物代谢网络中的一种关键辅因子。调节微生物胞内NADH的形式与浓度是定向改变和优化微生物细胞代谢功能, 实现代谢流最大化、快速化地导向目标代谢产物的重要手段之一。以下在详尽总结了NADH生理功能的基础上, 从生化工程(添加外源电子受体、不同氧化还原态底物及NAD合成前体物, 调节培养环境和氧化还原电势)和代谢工程(过量表达NADH代谢相关酶、缺失NADH竞争途径及引入NADH外源代谢途径)两方面分析、归纳了NADH代谢调控策略, 进而凝练出调控NADH/NAD+比率调节微生物细胞代谢功能研究方面亟待解决的3个科学问题及可能的解决途径。
|
| 关 键 词： | 形式与浓度 调控策略 工业微生物 |
| 收稿时间： | 2008-08-04 |
Manipulation of NADH metabolism in industrial strains |
| |
| Yi Qin,Zhiyao Dong,Liming Liu and Jian Chen. Manipulation of NADH metabolism in industrial strains[J]. Chinese journal of biotechnology, 2009, 25(2): 0161-0169 |
| |
| Authors: | Yi Qin Zhiyao Dong Liming Liu Jian Chen |
| |
| Affiliation: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China |
| |
| Abstract: | Nicotinamide adenine nucleotide (NADH), the key cofactor in the metabolic network, plays an essential role in biochemical reaction and physiological function of industrial strains. Manipulation of NADH availability and form is an efficient and easy way to redirect the carbon flux to the target metabolites in industrial strains. We reviewed the physiological function of NADH. Detailed strategies to manipulate NADH availability are addressed. NADH manipulation to enhance metabolic function of industrial strains was discussed and potential solutions were suggested. |
| |
| Keywords: | NADH |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
|
点击此处可从《生物工程学报》下载全文 |
|
