Effect of inorganic pyrophosphate on respiration and oxidative phosphorylation in higher plants |
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Authors: | Stanislaw Kowalczyk Piotr Maslowski |
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Institution: | Department of Biochemistry, Institute of Biology, Miko |
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Abstract: | In coupled mitochondria of maize, inorganic pyrophosphate has no effect on electron transport whereas it competitively inhibits state 3 (with addition of ADP) respiration. The degree of inhibition depends on the ADP concentration in the reaction medium. At 150 and 30O μM ADP, the inhibition constant (Ki) has a value of 1.1 × 10−4 M. Pyrophosphate either does not penetrate throucvh the membranes or penetrates through them in only very small amounts. It does not inhibit the exchange 32Pi Pi; however, it undergoes an exchange with ADP (2 nmol PPi/mg protein for 10 min at 30°). |
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Keywords: | Zea mays Gramineae corn inorganic pyrophosphate: respiratory control translocation |
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