Irrepressible,truncated Auxin Response Factors: Natural roles and applications in dissecting auxin gene regulation pathways |
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| Authors: | Wenzislava Ckurshumova Naden T Krogan Danielle Marcos Adriana E Caragea Thomas Berleth |
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| Institution: | Department of Cell and Systems Biology, University of Toronto; Toronto, ON Canada |
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| Abstract: | The molecularly well-characterized auxin signal transduction pathway involves two evolutionarily conserved families interacting through their C-terminal domains III and IV: the Auxin Response Factors (ARFs) and their repressors the Aux/IAAs, to control auxin-responsive genes, among them genes involved in auxin transport.1,2 We have developed a new genetic tool to study ARF function. Using MONOPTEROS (MP)/ARF5, we have generated a truncated version of MP (MPΔ),3 which has lost the target domains for repression by Aux/IAA proteins. Besides exploring genetic interactions between MP and Aux/IAAs, we used this construct to trace MP’s role in vascular patterning, a previously characterized auxin dependent process.4,5 Here we summarize examples of naturally occurring truncated ARFs and summarize potential applications of truncated ARFs as analytical tools. |
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