The Tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1 |
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| Authors: | Amalia Bar-Ziv Yael Levy Hagit Hak Anahit Mett Eduard Belausov Vitaly Citovsky Yedidya Gafni |
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| Institution: | 1.Institute of Plant Sciences; A.R.O.; The Volcani Center; Bet Dagan, Israel;2.The Mina and Everard Goodman Faculty of Life Sciences; Bar-Ilan University; Ramat-Gan, Israel;3.Department of Biological Chemistry; The Alexander Silberman Institute of Life Sciences; The Hebrew University of Jerusalem; Jerusalem, Israel;4.Department of Biochemistry and Cell Biology; State University of New York; Stony Brook, NY USA |
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| Abstract: | The V2 protein of Tomato yellow leaf curl geminivirus (TYLCV) is an RNA-silencing suppressor that counteracts the innate immune response of the host plant. However, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. This binding occurred both in vitro and in vivo, within living plant cells. The V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site. |
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| Keywords: | CYP1 papain-like cysteine protease protein interaction TYLCV V2 protein |
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