乙酰基亚硝基脲诱导小鼠突变的初步研究

目的　探索乙酰基亚硝基脲 (ENU)诱导小鼠突变的效率 ,筛查并获得能显性遗传的突变型小鼠。方法　采用 8～ 10周龄的雄性C57BL 6小鼠 33只、DBA 2小鼠 18只 ,腹腔注射ENU10 0mg kg ,每周一次共三次 ,与同品系母鼠配种 ,在后代小鼠中针对可见表型筛查突变个体。结果　处理雄鼠有 9至 13周的不育期 ;在已经筛查的12 4 1只小鼠中得到眼睛异常、多趾、少趾及腹部白斑、矮小等突变个体 6 1只 ,突变率约 5 % ;获得单基因显性遗传的突变品系 2种。结论　ENU为小鼠的强诱突变剂 ;通过诱变可以得到遗传突变小鼠 ,为建立人类疾病动物模型提供条件 ;大规模诱变实验对小鼠功能基因组的研究有重要意义

Preliminary Study of Mice Mutation Induced by ENU

Objective To study the efficiency of mice mutation induced by ENU. To screen and acquire the mutant mice with some new traits showing dominant heredity. Methods 33 C 57BL/6 and 18 DBA/2 male mice about 8-10 weeks old were injected intraperitoneally with a dose of ENU 100 mg/kg once a week and total three times. Mated with the same strain female mice, their progeny were screened for mutations. Results Treated males became transient sterility and the average length of sterile period was 9-13 weeks. In 1241 mice screened, 61 mice were produced with mutations, such as abnormal eyes, dwarf, white belly spot,and so on. The mutation percentage was about 5%. There were two mutation strains showing dominant heredity. Conclusion The mouse models for human diseases could be obtained by ENU-induced mutation. ENU mutagenesis on a large scale had important value for functional genome research.