Identification of a unique membrane receptor for adenosine 5',5"'-P1,P4-tetraphosphate |
| |
| Authors: | R H Hilderman M Martin J K Zimmerman E B Pivorun |
| |
| Affiliation: | Department of Biological Sciences, Clemson University, South Carolina 29634-1903. |
| |
| Abstract: | Adenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) has been implicated as a modulator of cell stress. We have performed binding studies which indicate that membranes from all tissues tested bind tritium-labeled Ap4A. The characteristics of Ap4A binding were determined on brain membrane homogenates after development of an optimized in vitro filter-binding assay. Ap4A binding is specific for adenylated dinucleotides and for the length of the phosphate bridge. A Kd of 0.71 microM for Ap4A was determined. |
| |
| Keywords: | |
|
|
