Spectrophotometric parameters of conformational states of hemoglobin and its complexes with ligands in the sturgeons |
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Authors: | A S Vasil’ev V I Luk’yanenko |
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Institution: | (1) Russian Academy of Sciences, Borok, Yaroslavl raion, Institute of Biology of Inner Waters, Russia |
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Abstract: | Parameters of carbo-, carboxy-, oxy-, deoxy-, and methemoglobin of the human and four species of sturgeons (sterlet, Russian
sturgeon, starred sturgeon, great sturgeon) were analyzed spectrophotometrically. It has been shown that in the absorption
spectra of all forms of hemoglobins there is only one Soret γ1 band due to the prosthetic group of the pigment; the wavelength of this band is undoubtedly associated with the hemoglobin
conformation. This permits the use of the band as an indicator parameter to control an initial state of the fish hemoglobin
solutions, the analysis of conformational states of the pigment macromolecules, and the study of hemoglobin derivatives (its
complexes with ligands). |
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