Effects of surfactants on cell growth and pigment production in suspension cultures ofPerilla frutescens |
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Authors: | J -J Zhong T Seki S -I Kinoshita T Yoshida |
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Institution: | (1) International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada-oka, 565 Suita-shi, Osaka, Japan |
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Abstract: | The effects of surfactants, adecanol LG-294 and silicone A, on anthocyanin accumulation and the growth ofPerilla frutescens cells in suspension cultures were studied. Production of the red pigment was remarkably reduced from about 1.9 g/l to 0.4 g/l by adecanol LG-294 at 0.06 ml/l but not by silicone A up to 0.4 ml/l. Several repeated shake-flask cultures also demonstrated no adverse effects of silicone A on the metabolite accumulation by the suspended cells. Furthermore, the addition of silicone A to a culture in a stirred bioreactor produced a three-fold higher growth rate and a seven-fold increase in anthocyanin compared with surfactant-free cultures. The improvement was due to the substantial reduction or prevention of foaming and of cell adhesion to the bioreactor wall. |
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Keywords: | Adecanol adhesion anthocyanin bioreactor foaming Perilla frutescens plant cell silicone surfactant |
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