Genomic Bacterial Artificial Chromosome Library of the Japanese Flounder Paralichthys olivaceus |
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| Authors: | Takayuki Katagiri Shuichi Asakawa Ikuo Hirono Takashi Aoki Nobuyoshi Shimizu |
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| Institution: | (1) Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan, JP;(2) Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan, JP |
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| Abstract: | We have constructed a genomic bacterial artificial chromosome (BAC) library from homozygous cloned Japanese flounder Paralichthys olivaceus using the pBAC-lac vector. This BAC library consists of about 49,100 clones and is deposited in 128 microtiter plates with 384 wells. The average
size of inserted DNA was calculated to be 165 kb. The BAC library was determined to cover 9 times the Japanese flounder haploid
genome. The Japanese flounder genomic BAC library will be useful for gene isolation as well as quantitative trait loci (QTL)
analysis.
Received March 1, 2000; accepted May 29, 2000. |
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| Keywords: | : Japanese flounder Paralichthys olivaceus bacterial artificial chromosome (BAC) high-density replica filter |
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