| 中华绒螯蟹遗传变异的RAPD分析 |
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| 引用本文： | 高志千,周开亚. 中华绒螯蟹遗传变异的RAPD分析[J]. 生物多样性, 1998, 6(3): 186-190. DOI: 10.17520/biods.1998028 |
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| 作者姓名： | 高志千 周开亚 |
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| 作者单位： | (南京师范大学生物多样性与分子进化研究室, 南京 210097) |
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| 基金项目： | 江苏省应用基础研究计划,江苏省水产局资助 |
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| 摘 要： | 用31个可重复性好的随机引物对中华绒螯蟹(Eriocheir sinensis)的辽河、瓯江和长江种群进行了RAPD分析。121个扩增片段中有27个多态片段。遗传距离指数（D）表明中华绒螯蟹种内遗传变异较低(D=0.004～0.063)。3个种群中，辽河种群(D=0.018)和瓯江种群(D=0.025)遗传变异较高，而长江种群遗传变异较低(D=0.013)；辽河种群和瓯江种群间遗传距离(D=0.023)小于它们与长江种群间的遗传距离(D值分别为0.028和0.032)。提示人类经济活动可能已使这2个种群发生了基因交流。
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| 关 键 词： | 中华绒螯蟹 遗传变异 RAPD |
| 收稿时间： | 1997-09-09 |
| 修稿时间： | 1998-02-04 |
Genetic variation of the Chinese mitten-handed crab (Eriocheir sinensis) populations detected by RAPD analysis |
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| GAO Zhi-Qian,ZHOU Kai-Ya. Genetic variation of the Chinese mitten-handed crab (Eriocheir sinensis) populations detected by RAPD analysis[J]. Biodiversity Science, 1998, 6(3): 186-190. DOI: 10.17520/biods.1998028 |
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| Authors: | GAO Zhi-Qian ZHOU Kai-Ya |
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| Affiliation: | Biodiversity and Molecular Evolution Laboratory , Nanjing Normal University , Nanjing 210097 |
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| Abstract: | RAPD was employed to assess the genetic variation among Eriochei r sinensis populations of theYangtze , Liaohe and Oujiang river systems. 31 random reproducible primers revealed 121 amplified fragmentsin which 27 were polymorphic. Intraspecific genetic variation was low ( D = 01004～01063) . Intrapopulationgenetic variation of Liaohe population and Oujiang population were higher than that of Yangtze populationwhile the interpopulation variation between Liaohe and Oujiang populations was lower than that between eitherof them and Yangtze population. The results suggest that the human economic activities have probably causedgene exchanges between the two populations. |
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| Keywords: | Eriocheir sinensis genetic variation RAPD |
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