首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Evidence of endoplasmic reticulum-related Ca2+ ATPase in human microvascular endothelial cells
Authors:A Bikfalvi  J Enouf  R Bredoux  A Lompre  E Dupuy  N Bourdeau  S Levy-Toledano  G Tobelem
Institution:Inserm U 150 and CNRS URA 184, H?pital Lariboisière, Paris, France.
Abstract:We have demonstrated by immunological and molecular methods the presence of a reticulum endoplasmic-related Ca2+-ATPase in human omental microvascular endothelial cells (HOME cells). HOME cells reacted positively with a previously characterized sarcoplasmic reticulum Ca2+-ATPase antibody as demonstrated by indirect immunofluorescence. Western blotting revealed that the antibody recognized a 95-100 kDa protein. 35S-Metabolic labeling led to the detection of a similar protein with which the purified sarcoplasmic reticulum Ca2+-ATPase competed. Dot-blotting experiments indicated that a substantial amount of Ca2+-ATPase was present in HOME cell membranes. In addition, Northern blot analysis using a cDNA probe from cardiac sarcoplasmic reticulum showed the presence of mRNA species of 4-kb. As these experiments were conducted in comparison with cell types with well-defined Ca2+-ATPase in HOME cells.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号