Localization of claudin-5 and ZO-1 in rat spleen sinus endothelial cells |
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Authors: | Kiyoko Uehara Akira Uehara |
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Institution: | (1) Department of Cell Biology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-0180, Japan;(2) Department of Physiology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka 814-0180, Japan |
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Abstract: | Splenic sinus endothelial cells, which adhere through tight and adherens junctions, regulate the passage of blood cells through
the splenic cord. The objective of this study was to assess the localization of tight junctional proteins, claudin-5 and ZO-1
in the sinus endothelial cells of rat spleen and to characterize spatial and functional relationships between tight and adherens
junctions. Immunofluorescence microscopy of tissue cryosections demonstrated that claudin-5, ZO-1, and α-catenin were distinctly
localized in the junctional regions of adjacent endothelial cells. Immunogold electron microscopy demonstrated claudin-5 localized
in the tight-junctional fused membranes of adjacent endothelial cells. Immunogold labeling for ZO-1 was localized not only
in the tight-junctional-fused membranes of endothelial cells but also in the junctional membrane. α-Catenin was intermittently
localized along the juxtaposed junctional membranes of adjacent endothelial cells. Double-staining immunogold microscopy for
claudin-5 and ZO-1, claudin-5 and VE-cadherin, ZO-1 and VE-cadherin, and ZO-1 and α-catenin demonstrated that ZO-1 was closely
localized to VE-cadherin and α-catenin in their juxtaposed membranes of endothelial cells. Thus, ZO-1 might play an important
role in regulating the cell–cell junctions of sinus endothelial cells for blood–cell passage through splenic cords.
This work was supported by a Grant-in-Aid for Scientific Research (C), Japan. |
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Keywords: | Claudin-5 ZO-1 α -Catenin Vascular endothelial cadherin Sinus endothelial cells |
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