Effect of Carbon Source on Production of α-L-Arabinofuranosidase by Aureobasidium pullulans |
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Authors: | Badal C Saha Rodney J Bothast |
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Institution: | (1) Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA , US |
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Abstract: | A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produced α-L-arabinofuranosidase (α-L-AFase) when grown in liquid culture on sugar beet arabinan, wheat arabinoxylan,
L-arabinose, L-arabitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalactan. L-Arabinose was most effective
for production of both whole-broth and extracellular α-L-AFase activity, followed by L-arabitol. Oat spelt xylan, sugar beet
arabinan, xylose, xylitol, and wheat arabinoxylan were intermediate in their ability to support α-L-AFase production. Lower
amounts of enzyme activity were detected in corn fiber- and arabinogalactan-grown cultures.
Received: 16 April 1998 / Accepted: 17 June 1998 |
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