Polymorphism of the prion protein gene (<Emphasis Type="Italic">PRNP</Emphasis>) in two Chinese indigenous cattle breeds |
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Authors: | L H Qin Y M Zhao Y H Bao W L Bai J Chong G L Zhang J B Zhang Z H Zhao |
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Institution: | (1) College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China;(2) Agricultural Science Academy of Jilin Province, Gongzhuling, 136100, China;(3) College of Basic Medical, Xinxiang Medical University, Xinxiang, 453003, China;(4) College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China;(5) Laboratory Animal Center, Jilin University, Changchun, 130062, China; |
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Abstract: | Prion protein (PRNP) gene has been located at position q17 of chromosome 13 in cattle. The polymorphisms of PRNP gene might be associated with BSE susceptibility. In the present work, we investigated the polymorphisms of PRNP gene, including SNP in exon 3, 23-bp indel in promoter region, 12-bp indel in intron 1 in 2 Chinese indigenous cattle breeds
of northeast China. Eighty-six animals from Yanbian (34) and Chinese Red Steppes (52) were genotyped at PRNP locus by analyzing genomic DNA. A total of 4 single nucleotide polymorphism (SNP) sites were revealed in the PRNP gene exon 3 of the 2 cattle breeds investigated. Three of these SNPs were non-synonymous mutations that resulted in the amino
acid exchanges (K119N, S154N, and M177V), and one is silent nucleotide substitutions (A234G). The two amino acid mutations
of S154N and M177V were detected only in Yanbian with a very low frequency (0.0147), and they appears to be absent in Chinese
Red Steppes. The average gene heterozygosity (H
e), effective allele numbers (N
e), Shannon’s information index (I) and polymorphism information content (PIC) were 0.3088, 1.5013, 0.3814 and 0.2000 in Yanbian, respectively, being relatively
higher than that of Chinese Red Steppes (0.2885, 1.4985, 0.3462 and 0.1873, respectively). In 23-bp indel and 12-bp indel
loci, three different genotypes were identified in both Yanbian and Chinese Red Steppes breeds. Based 23- and 12-bp indels,
four haplotypes was constructed in the 2 Chinese cattle breeds, of which the 23-bp (−)/12-bp (−) was main haplotypes accounting
for more than 50% of the total in both Yanbian and Chinese Red Steppes breeds. These results might be useful in understanding
the genetic characteristics of PRNP gene in Chinese indigenous cattle breeds. |
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