几种植物原生质体的扫描电镜观察

扫描电镜观察表明,分离自马铃薯、萱草。甘蔗、木薯和落花生等不同植物和组织的原生质体表面呈现不同程度的凹凸不平。马铃薯叶肉原生质体表面较粗糙,其余四种植物叶肉、幼茎或子叶原生质体稍光滑。有的原生质体显现不同程度的凹陷现象。有的原生质体表面尚残留有未完全水解的胞壁碎片。在木薯幼茎原生质体制备物中见有呈“裂片”状的球形结构。原生质体表面扫描图象的差异似与不同种植物有关,与组织源不同更有密切关系。 原生质体镀膜前,涂布于已镀膜的盖玻片支持物上的原生质体很少或无凹陷现象,涂布于已镀膜的双面胶支持物上的原生质体凹陷严重。

SCANNING ELECTRON MICROSCOPIC OBSERVATION ON PROTOPLASTS OF FIVE SPECIES

The scanning electron microscopic observation on protoplasts which were isolated from mesophyll of potato (Solanum tuberosum), orange daylily (Hemer-ocallis fulva L. ) and sugar cane (Saccharum officinarum), from young stemlet of cassava (Manihot esculenta Crantz) and young cotyledon of peanut (Arachis hypogae(?) L.) are indicated that the surfaces of protoplasts were full of bumps and holes in varying degrees. Among them the surfaces of potato mesophyll protoplasts were rougher, but the mesophyll protoplast surfaces of orange daylily and sugar cane, the young stemlet protoplast surface of cassava and the young cotyledon protoplast surface of peanut were all relatively smooth. Some protoplast surface showed partial and slight sunk. Partial broken surface could be easily occurred in the preparing process of young protoplast isolated from peanut young cotyledon, thereby the protoplast showed many small holes. Some protoplast surface had still the remainder of cell wall. The "labate form" spheroidal structure was observed in the cassava stemlet protoplasts preparation. The difference of scanning electron microscopic pattern of potoplasts surfaces seems bearing relation to the different plant species, but in close relationship with the difference of tissuesThe protoplasts could easily aggregate each other, but slightly, when the protoplasts were smeared on the sheet glass before vacuum coating. Contrary, the protoplasts were well distributed but sunk sericusly,when the protoplasts were smeared on the double-edged film before vacuum coating.