| 利用RAPD分子标记对三组三系杂交水稻及亲本的遗传分析和鉴定 |
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| 引用本文： | 毛加宁,段世华,李绍清,朱英国.利用RAPD分子标记对三组三系杂交水稻及亲本的遗传分析和鉴定[J].遗传,2002,24(3):283-287. |
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| 作者姓名： | 毛加宁 段世华 李绍清 朱英国 |
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| 作者单位： | 武汉大学植物发育生物学教育部重点实验室,武汉大学生命科学学院遗传研究所，武汉 430072
The Key Laboratory of MOE for Plant Development Biology and Institute of Genetics,College of Life Sciences,Wuhan University,Wuhan 430072,China |
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| 基金项目： | 国家自然科学基金 (批准号 :39770 45 5 ) |
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| 摘 要： | 利用RAPD技术，从248个随机寡聚核苷酸（10bp）中筛选出13个引物能在供试的三组三系杂交水稻及亲本间扩增出43条稳定性较好的多态性片段，其中6个引物能在供试材料间扩增出20个强的多态性标记。利用这些标记能有效地区分各组合中不育系、保持系、恢复系和F1，并能看出各组合中不育系与保持系、不育系与恢复系、F1与亲本间的遗传关系。
Abstract:A total of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) techniques with the genome DNA of three groups of three-line hybrid rice and their parents.Thirteen primers produced 43 polymorphism fragments.Six primers of them produced 20 obviously repeatable polymorphic markers among rice lines tested.Using this RAPD markers,the hybrid rice combinations (sterile-line,maintainer-line,restorer-line and F1)can be effectively identified,and the genetic relationship among them can be shown.
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| 关 键 词： | RAPD 杂交水稻 RAPD 鉴定 Key words 遗传关系 |
| 文章编号： | 0253-9772(2002)03-0283-05 |
| 修稿时间： | 2001年6月29日 |
Genetic Analysis and Identification of Three Groups Three-line Hybrid Rice and Their Parents by RAPD Markers |
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| MAO Jia ning,DUAN Shi hua,LI Shao qing,ZHU Ying guo.Genetic Analysis and Identification of Three Groups Three-line Hybrid Rice and Their Parents by RAPD Markers[J].Hereditas,2002,24(3):283-287. |
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| Authors: | MAO Jia ning DUAN Shi hua LI Shao qing ZHU Ying guo |
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| Institution: | The Key Laboratory of MOE for Plant Development Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China. |
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| Abstract: | A total of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) techniques with the genome DNA of three groups of three-line hybrid rice and their parents.Thirteen primers produced 43 polymorphism fragments.Six primers of them produced 20 obviously repeatable polymorphic markers among rice lines tested.Using this RAPD markers,the hybrid rice combinations (sterile-line,maintainer-line,restorer-line and F 1)can be effectively identified,and the genetic relationship among them can be shown. |
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| Keywords: | RAPD Hybrid rice Genetic relationship Identification |
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