| 生物酶法催化瓦伦西亚烯生成圆柚酮 |
| |
| 引用本文： | 孟飞,俞春娜,李海峰,谢恬.生物酶法催化瓦伦西亚烯生成圆柚酮[J].生物技术通报,2012(8):194-198. |
| |
| 作者姓名： | 孟飞 俞春娜 李海峰 谢恬 |
| |
| 作者单位： | 1. 杭州师范大学生物医药与健康研究中心,杭州311121;杭州师范大学生命与环境科学学院,杭州310036
2. 杭州师范大学生物医药与健康研究中心,杭州,311121 |
| |
| 基金项目： | 国家自然科学基金项目，杭州市科技发展计划项目，国家重大科技专项子课题 |
| |
| 摘 要： | 在体外,利用野生型CYP450BM-3对瓦伦西亚烯进行催化,酶-底物复合物催化NADPH氧化的速率为31±1.0 nmol(nmol P450)-1min-1,但催化产物中没有检测到圆柚酮的生成。突变体R47L/Y51F/F87A与底物复合物催化NADPH氧化的速率高于野生型,为79±6.5 nmol(nmol P450)-1min-1,并在催化产物中检测到圆柚酮的生成,但其产物选择性较差,圆柚酮的含量仅占总产物的6.8%。与此同时,检测了另一个突变体A74G/F87V/L188Q对瓦伦西亚烯的催化效果,发现其与底物复合物对NADPH的氧化速率与突变体R47L/Y51F/F87A相当,但产物中圆柚酮的比率更高,达8.0%。
|
| 关 键 词： | 圆柚酮 瓦伦西亚烯 细胞色素 P450BM-3突变体 生物催化 |
Production of (+) -Nootkatone from (+) -Valencene by Biocatalysis |
| |
| Meng Fei
,
Yu Chunna
,
Li Haifeng
,
Xie Tian.Production of (+) -Nootkatone from (+) -Valencene by Biocatalysis[J].Biotechnology Bulletin,2012(8):194-198. |
| |
| Authors: | Meng Fei Yu Chunna Li Haifeng Xie Tian |
| |
| Institution: | 1(1Centre for Biomedicine and Health,Hangzhou Normal University,Hangzhou 311121;2School of Life and Environmental Science,Hangzhou Normal University,Hangzhou 310036) |
| |
| Abstract: | Wild-type CYP450BM-3 showed NADPH oxidation rate of 31±1.0 nmol(nmol P450)-1min-1 in vitro,however,(+)-nootkatone could not be detected in the products.The R47L/Y51F/F87A mutant had higher activity of 79±6.5 nmol(nmol P450)-1min-1.However,it was much less selective and(+)-nootkatone constituted 6.8% of the total products.Meanwhile,another mutant A74G/F87V/L188Q was found to have similar NADPH oxidation activity with the R47L/Y51F/F87A mutant but a higher(+)-nootkatone ratio of 8.0% was generated. |
| |
| Keywords: | (+)-Nootkatone(+)-Valencene Cytochrome P450BM-3 Mutant Biocatalysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
