| 香蕉枯萎病菌果胶甲基酯酶基因的克隆与生物信息学分析 |
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| 引用本文： | 董章勇,王振中. 香蕉枯萎病菌果胶甲基酯酶基因的克隆与生物信息学分析[J]. 生物技术通报, 2012, 0(6): 111-115 |
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| 作者姓名： | 董章勇 王振中 |
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| 作者单位： | 1. 仲恺农业工程学院,广州510225;华南农业大学植物病理生理学研究室,广州510642
2. 华南农业大学植物病理生理学研究室,广州,510642 |
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| 基金项目： | 国家自然科学基金，公益性行业(农业)科研专项 |
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| 摘 要： | 旨在了解香蕉枯萎病菌(Fusarium oxysporumf.sp.cubense)4号生理小种(FOC4)PME基因序列特征,根据同源物种PME相关序列设计引物,利用PCR和RT-PCR技术,克隆FOC4序列基因和开放阅读框,命名为Foc4Pme。结果表明,所获得的PME基因均含有2个内含子和3个外显子,990 bp的片段,编码329个氨基酸。预测编码蛋白有信号肽,具有1个功能位点,其分子质量和等电点分为34.894 8 kD和9.17,该蛋白为稳定存在的蛋白。该蛋白疏水性最大值为2.022,最小值为-2.156,大部分区域为亲水区。该基因编码的蛋白具有一定保守性,进化上与镰刀菌亲缘关系最近。
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| 关 键 词： | 果胶甲基酯酶 香蕉枯萎病 香蕉枯萎病菌 生物信息学分析 |
Cloning and Bioinformatic Analysis of Pectin Methylesterase Gene from Fusarium oxyporum f.sp.cubense |
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| Dong Zhangyong , Wang Zhenzhong. Cloning and Bioinformatic Analysis of Pectin Methylesterase Gene from Fusarium oxyporum f.sp.cubense[J]. Biotechnology Bulletin, 2012, 0(6): 111-115 |
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| Authors: | Dong Zhangyong Wang Zhenzhong |
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| Affiliation: | 1Zhongkai University of Agriculture and Engineering,Guangzhou 510225;2Laboratory of Physiological Plant Pathology,South China Agricultural University,Guangzhou 510642) |
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| Abstract: | To understand the Fusarium oxysporum f.sp.cubense race 4(FOC4) pectin methylesterase(PME) gene sequence features,a pair of primer that according to the homologous gene sequence documented in GenBank was designed to amplify the PME gene.Using PCR and RT-PCR,the genome and open reading frame(ORF) from FOC4 was successfully amplified and named it Foc4Pme.The gene included 2 introns and 3 exons.The ORF encoded 329 amino acid polypeptide with 34.894 8 kD of calculated molecular weight and 9.17 of pI.The protein hydrophobic maximum 2.022,minimum value is-2.156,most of the region for the hydrophilic areas.The protein with evolutionary conservative and got the closest relatives with Fusarium. |
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| Keywords: | Pectin methylesterase Fusarium wilt Fusarium oxyporum f.sp.cubense Bioinformatic analysis |
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