| HtrA2转基因鼠的建立与鉴定 |
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| 引用本文： | 柳明杰,沈雁飞.HtrA2转基因鼠的建立与鉴定[J].生物技术通报,2012(7):93-96. |
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| 作者姓名： | 柳明杰 沈雁飞 |
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| 作者单位： | 辽宁医学院免疫病原生物学教研室,锦州,121001 |
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| 基金项目： | 辽宁省科技厅博士启动项目，辽宁省教育厅科研项目，辽宁医学院博士科研启动基金 |
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| 摘 要： | 旨在建立神经特异性过表达HtrA2转基因鼠。通过质粒重组技术构建神经特异性表达HtrA2质粒pNSE-HtrA2,利用EcoR I及PvuI切割pNSE-HtrA2获得转基因。显微注射转基因到FVB/N受精卵并移植到假孕鼠的输卵管中发育,获得的转基因鼠利用Western blot鉴定特异性过表达情况。结果显示,成功建立神经特异性过表达HtrA2转基因鼠,神经特异性过表达HtrA2达到5.4倍。本研究建立了神经特异性过表达HtrA2转基因鼠,可用于HtrA2在神经系统的作用研究。
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| 关 键 词： | 转基因 过表达 HtrA2 转染 原代培养 |
Construction and Determination of HtrA2 Transgenic Mice |
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| Liu Mingjie
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Shen Yanfei.Construction and Determination of HtrA2 Transgenic Mice[J].Biotechnology Bulletin,2012(7):93-96. |
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| Authors: | Liu Mingjie Shen Yanfei |
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| Institution: | Liu Mingjie Shen Yanfei(Department of Immunology and Microbiology,Liaoning Medical University,Jinzhou 121001) |
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| Abstract: | It was to construct transgenic mice that specific over-expression of HtrA2 in neurons.The recombinant plasmid pNSE-HtrA2 that containing human HtrA2 cDNA downstream of NSE promoter was constructed and digested with EcoR I and Pvu I to obtain 4.3 kb transgene.Transgenic mice were obtained by microinjecting of transgene and specific over-expression of HtrA2 was detected by Western blot.Result showed the recombinant plasmid pNSE-HtrA2 was successfully constructed.Transgenic mice with specific-overexpression of HtrA2 up to 5.4 times comparison with controls were successfully obtained.Transgenic mice with specific over-expression of HtrA2 were successfully obtained.Functions of HtrA2 in neurons can be further detected using constructed transgenic mice. |
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| Keywords: | Transgene Over-expression HtrA2 Transfection Primary culture |
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