| 大豆种质资源SRAP分子标记中的引物筛选 |
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| 引用本文： | 王芳,徐翔,李领川,聂卉,李晓静,周延清.大豆种质资源SRAP分子标记中的引物筛选[J].生物技术通报,2012(7):83-87. |
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| 作者姓名： | 王芳 徐翔 李领川 聂卉 李晓静 周延清 |
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| 作者单位： | 1. 中州大学实验管理中心,郑州,450044
2. 河南师范大学生命科学学院,新乡,453007 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 以113个大豆栽培品种和20个野生品种为材料,从288对引物组合中筛选出12对多态性丰富、条带清晰、可重复性好的SRAP引物组合。用筛选出的12对引物组合对大豆品种进行PCR扩增,获得了带型丰富和清晰可辨的DNA的PAGE指纹图谱;共扩增出251条谱带,其中多态性条带220条,多态性谱带比率为87.6%,平均每个引物扩增出18.3条谱带。结果显示,所筛选出的12对引物组合可以有效的应用于大豆种质资源的SRAP分析。
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| 关 键 词： | 大豆 SRAP标记 引物筛选 |
Primer Screening on Germplasm Resources of Glycine max(L.)Merr.with SRAP Molecular Marker |
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| Wang Fang
,
Xu Xiang
,
Li Lingchuan
,
Nie Hui
,
Li Xiaojing
,
Zhou Yanqing.Primer Screening on Germplasm Resources of Glycine max(L.)Merr.with SRAP Molecular Marker[J].Biotechnology Bulletin,2012(7):83-87. |
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| Authors: | Wang Fang Xu Xiang Li Lingchuan Nie Hui Li Xiaojing Zhou Yanqing |
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| Institution: | 1Experiment Administration Center,Zhongzhou University,Zhengzhou 450044;2College of Life Sciences,Henan Normal University,Xinxiang 453007) |
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| Abstract: | In this study,12 primer combinations of SRAP with characteristics of rich polymorphism,clear bands and good repeatability were selected from 288 primer combinations for 113 accessions of cultivated soybean(Glycine max) and 20 accessions of wild soybean(Glycine soja).The 12 primer combinations were used in the PCR amplification,and the abundant and distinct DNA finger printings of PAGE were obtained.251 DNA bands were obtained,220 of which bands were polymorphic,amounted to 87.6% of the total amplified bands,and 18.3 bands were obtained with each primer,averagely.Result showed that the selected 12 primer combinations could be effectively applied to SRAP analysis of the germplasm resources of Glycine max(L.) Merr.. |
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| Keywords: | Glycine max(L )Merr SRAP marker Primer screening |
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