| 石油污染土壤中植物根际促生菌的筛选及特性分析 |
| |
| 引用本文： | 方芳,刘佳莉,史煦涵,陈红艳,郭长虹.石油污染土壤中植物根际促生菌的筛选及特性分析[J].生物技术通报,2012(6):154-158. |
| |
| 作者姓名： | 方芳 刘佳莉 史煦涵 陈红艳 郭长虹 |
| |
| 作者单位： | 哈尔滨师范大学生命科学与技术学院分子细胞遗传与遗传育种黑龙江省重点实验室,哈尔滨,150025 |
| |
| 基金项目： | 国家自然科学基金，黑龙江省自然科学基金，黑龙江省研究生科技创新项目 |
| |
| 摘 要： | 以1-氨基环丙烷-1-羧酸(ACC)为唯一氮源,从黑龙江省大庆地区石油污染土壤的狼尾草根际土壤中分离筛选出2株产ACC脱氨酶的细菌,F4-1和F4-2。对分离的菌株进行生理生化和16S rDNA序列鉴定,确定F4-1为肠杆菌属(Enterobactersp.),F4-2为克雷伯菌属(Klebsiellasp.)。菌株F4-1的ACC脱氨酶活性为(1.40±0.17)μmolα-KA.(mg Pr.h)-1,高于F4-2的(1.03±0.03)μmolα-KA.(mg Pr.h)-1。随着L-Trp浓度的增加,菌株F4-1和F4-2的吲哚乙酸(IAA)合成量相应增加,总体上看F4-1的IAA合成能力高于F4-2。F4-1合成嗜铁素的能力也高于F4-2。
|
| 关 键 词： | 石油 植物根际促生菌(PGPR) ACC脱氨酶 IAA 植物修复 |
Isolation and Characterization of PGPR from Petroleum-contaminated Soil |
| |
| Fang Fang
,
Liu Jiali
,
Shi Xuhan
,
Chen Hongyan
,
Guo Changhong.Isolation and Characterization of PGPR from Petroleum-contaminated Soil[J].Biotechnology Bulletin,2012(6):154-158. |
| |
| Authors: | Fang Fang Liu Jiali Shi Xuhan Chen Hongyan Guo Changhong |
| |
| Institution: | Fang Fang Liu Jiali Shi Xuhan Chen Hongyan Guo Changhong (Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,College of Life Science and Technology, Harbin Normal University,Harbin 150025) |
| |
| Abstract: | We took use of the oil polluted rhizosphere soil of Pennisetum alopecuroides(L.)Spreng.in Daqing City in Heilongjiang province area,1-aminocyclopropane-1-carboxylic acid as the sole nitrogen source,two plant growth-promoting bacteria strains F4-1and F4-2 which can product ACC deaminase were isolated.According to morphology and physiological biochemical characteristics and 16S rDNA,the strain F4-1 was identified as Enterobacter sp.and F4-2 was confirmed as Klebsiella sp..The ACC deaminase activity of F4-1 was(1.40±0.17) μmol α-KA·(mg Pr·h)-1,and it was higher than F4-2 which was(1.03±0.03) μmol α-KA·(mg Pr·h)-1.With the concentration of L-Trp increased,the IAA synthesis of both F4-1 and F4-2 were increased,and the IAA and siderophore synthesis of F4-1 was higher than F4-2. |
| |
| Keywords: | Petroleum Plantgrowth-promoting rhizobacteria(PGPR) 1-aminocyclopropane-1-carboxylic acid deaminase IAA Phytoremediation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
