| 蛋白质二级结构的真空紫外圆二色性研究 |
| |
| 引用本文： | 林波海. 蛋白质二级结构的真空紫外圆二色性研究[J]. 生物化学与生物物理进展, 1994, 21(1): 67-69 |
| |
| 作者姓名： | 林波海 |
| |
| 作者单位： | 中国科学院生物物理研究所 |
| |
| 摘 要： | 利用同步辐射真空紫外圆二色谱仪和特制的样品池，测定溶液中蛋白质的真空紫外圆二色谱，测定波长低至175nm，并应用一种新的计算法分析计算了蛋白质5种二级结构的含量，所得结果与用X射线衍射法测定的结果一致.讨论了获得好的真空紫外圆二色谱的几个重要因素.结果表明，真空紫外圆二色法是目前测定溶液中蛋白质二级结构的较好方法之一.
|
| 关 键 词： | 真空紫外圆二色谱 同步辐射 蛋白质二级结构 |
| 收稿时间： | 1993-02-02 |
| 修稿时间： | 1993-04-06 |
Vacuum Ultraviolet Clrcular Dichroism Studies on Secondary Structure of Proteins |
| |
| Lin Bo-hai. Vacuum Ultraviolet Clrcular Dichroism Studies on Secondary Structure of Proteins[J]. Progress In Biochemistry and Biophysics, 1994, 21(1): 67-69 |
| |
| Authors: | Lin Bo-hai |
| |
| Affiliation: | Institute of Biophysics, Academia Sinica |
| |
| Abstract: | Vacuum ultraviolet circular dichroism (VUV-CD) spectra of proteins in solution have been measured using synchrotron radiation vacuum ultraviolet circular dichroism spectrometer and a special cell. The measurement wavelength is down to 175nm. A new calculation method has been applied for calculating the content of five kinds of secondary structures of proteins. Their results are coincident with that from X-ray diffraction method. In order to get good VUV-CD spectra, several important factors have been discussed. The experiments show that so far. VUV-CD analysis is one of the favorable method for secondary structure studies of proteins. |
| |
| Keywords: | vacuum ultraviolet circular dichroism synchrotron radiation Secondary structure of proteins |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
