| 核糖体DNA的内转录间隔区序列标记在真菌分类鉴定中的应用 |
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| 引用本文： | 林剑伟,阙友雄,陈天生,许莉萍,张木清.核糖体DNA的内转录间隔区序列标记在真菌分类鉴定中的应用[J].生物技术通讯,2007,18(2):292-294. |
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| 作者姓名： | 林剑伟 阙友雄 陈天生 许莉萍 张木清 |
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| 作者单位： | 福建农林大学,农业部甘蔗生理生态与遗传育种重点开放实验室,福建,福州,350002 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;引进国际先进农业科技计划(948计划) |
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| 摘 要： | 传统的真菌分类主要根据真菌菌株的形态特征、生长特性与生理生化指标进行,而分子生物学技术的发展提升了真菌分类鉴定研究的手段。真菌核糖体DNA内转录间隔区(ITS)在进化上比编码区快,种内的不同菌株之间高度保守,但在种间变化极大,故可为真菌学的研究提供丰富的遗传信息。简要综述了ITS序列分析技术在真菌分类鉴定中的应用现状、相关问题及前景。
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| 关 键 词： | 核糖体DNA 内转录间隔区 分类 真菌 |
| 文章编号： | 1009-0002（2007）02-0292-03 |
| 修稿时间： | 2006-07-20 |
Application of Ribosomal DNA Internal Transcribed Spacer in Fungi Taxonomy |
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| LIN Jian-wei,QUE You-xiong,CHEN Tian-sheng,XU Li-ping,ZHANG Mu-qing.Application of Ribosomal DNA Internal Transcribed Spacer in Fungi Taxonomy[J].Letters in Biotechnology,2007,18(2):292-294. |
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| Authors: | LIN Jian-wei QUE You-xiong CHEN Tian-sheng XU Li-ping ZHANG Mu-qing |
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| Abstract: | Traditional classification methods of fungi are mainly based on their morphological characters,growth characters or physiological and biochemical indexes.Along with the fast development of molecular biology technology,the method of classification and identification of fungi was developed.Among them,ribosomal DNA internal transcribed spacers(ITS),evolves faster than coding sequence region,is highly conservative within species,but with great difference between species.Thus,it can provide abundant genetic information for the research of mycology.In this paper,current status of ITS sequence analysis in the field of fungal classification and identification,discussion of related problems and the prospect were briefly summarized. |
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| Keywords: | ribosomal DNA internal transcribed spacer classification fungi |
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