| 富含多糖草莓果实总RNA提取方法的改进 |
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| 引用本文： | 周波,张旸,李玉花. 富含多糖草莓果实总RNA提取方法的改进[J]. 生物技术通讯, 2004, 15(1): 48-50 |
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| 作者姓名： | 周波 张旸 李玉花 |
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| 作者单位： | 东北林业大学,花卉生物工程研究所,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金(30170785) |
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| 摘 要： | 以草莓果实为模式实验材料，将Chomczynski提出的常规RNA提取方法与Kenneth等提出的改进方法相结合，并做了进一步改进，建立了一种简单实用的从富舍多糖的植物材料中提取总RNA的方法。先利用冷的丙酮去除色素类物质，再利用乙二醇丁醚去除多糖，从而有效克服了从富含色素和多糖娄物质的植物材料中提取RNA的困难；获取的RNA样品在纯度和浓度上都可以满足PCR及Northern杂交等分子生物学实验的要求。
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| 关 键 词： | 多糖 草莓果实 总RNA 提取方法 |
| 文章编号： | 1009-0002(2004)01-0048-03 |
| 修稿时间： | 2003-06-23 |
Improvement of extraction method of total RNA from strawberry fruit with abundant polymeric carbohydrates |
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| ZHOU Bo,ZHANG Yang,LI Yu-hua Research Institute of Flower Biotechnology,Northest Forestry University,Harbin,China. Improvement of extraction method of total RNA from strawberry fruit with abundant polymeric carbohydrates[J]. Letters in Biotechnology, 2004, 15(1): 48-50 |
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| Authors: | ZHOU Bo ZHANG Yang LI Yu-hua Research Institute of Flower Biotechnology Northest Forestry University Harbin China |
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| Affiliation: | ZHOU Bo,ZHANG Yang,LI Yu-hua Research Institute of Flower Biotechnology,Northest Forestry University,Harbin150040,China |
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| Abstract: | A simple and convenient extraction method of total RNA from plant materials with abundant polymeric carbohy-drates was established by using strawberry fruit as model experimental material.It was a further improved method of com-bining the conventional total RNA extraction method developed by Chomczynski with an improved extraction method devel-oped by Kenneth.The key improved points are clearing pigment first by cooled acetone,and then eliminating polymeric carbohydrates by ethylenglycol monobutyl ether.It was effectively overcome the obstacle for total RNA extraction from plant materials with abundant pigment and polymeric carbohydrates.The purity and concentration of extracted total RNA can meet the demand of molecular biology experiment such as PCR and Northern hybridization. |
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| Keywords: | polymeric carbohydrates strawberry fruit total RNA extraction methods |
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