Initial characterization of the carbohydrate structure of MCP-1 |
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| Authors: | Y Jiang L A Tabak A J Valente D T Graves |
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| Affiliation: | Department of Oral Biology, Boston University Medical Center, MA 02118. |
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| Abstract: | MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-beta 1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration. |
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