1,25,26-trihydroxyvitamin D3: isolation, identification, and biological activity |
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Authors: | Y Tanaka H K Schnoes C M Smith H F DeLuca |
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Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706 USA |
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Abstract: | A polar metabolite of vitamin D3 has been produced in vitro from either 1,25-dihydroxyvitamin D3 incubated with kidney homogenate from vitamin D-supplemented chickens or from 25,26-dihydroxyvitamin D3 incubated with vitamin D-deficient chicken kidney homogenate. This compound was isolated in pure form and identified as 1,25,26-trihydroxyvitamin D3 by ultraviolet absorption spectrophotometry and mass spectrometry. Furthermore, its periodate cleavage product comigrates with synthetic 1α-hydroxy-25-keto-27-norvitamin D3 on high-performance liquid chromatography. The 1,25,26-trihydroxyvitamin D3 is 0.1-0.01 as active as 1,25-dihydroxyvitamin D3 in the stimulation of intestinal calcium transport and bone calcium mobilization. |
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Keywords: | To whom all correspondence should be addressed |
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