Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from <Emphasis Type="Italic">Bacillus</Emphasis><Emphasis Type="Italic">circulans</Emphasis>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from Bacilluscirculans

Authors:	A.?Rosso,S.?Ferrarotti,M.V.?Miranda,N.?Krymkiewicz,B.C.?Nudel,O.?Cascone author-information" > author-information__contact u-icon-before" > mailto:ocasco@ffyb.uba.ar" title=" ocasco@ffyb.uba.ar" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Departamento de Ciencias Básicas, Universidad Nacional de Luján, Rutas 5 y 7, 6700 Luján, Buenos Aires, Argentina;(2) Cátedra de Microbiología Industrial y Biotecnología. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina

Abstract:	Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM α-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37.

Keywords:	affinity Bacillus circulans cyclodextrin glycosyltransferase purification
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