Symplastic communication between the central cell and the egg apparatus cells in the embryo sac of Torenia fournieri Lind. before and during fertilization |
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Authors: | Yu-Zhen Han Bing-Quan Huang Sze-Yong Zee Ming Yuan |
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Institution: | (1) Department of Botany, The University of Hong Kong, Hong Kong, CN;(2) Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China, CN |
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Abstract: | Various membrane-impermeable, water-soluble fluorescent tracers with different molecular weights were microinjected into
the central cell of the embryo sac of Torenia fournieri Lind. before and during fertilization. Before anthesis, there was high symplastic permeability between the central cell and
the egg apparatus cells. In this stage, fluorescent tracers up to 10 kDa could pass from the central cell into the egg apparatus
cells, whereas those with larger molecular weight remained in the central cell. As the embryo sac matured, symplastic permeability
decreased such that 2 d after anthesis only tracers less than 3 kDa could spread from the central cell into the egg cell.
There appeared to be no symplastic permeability between the primary endosperm and zygote after fertilization, since tracers
as small as 521 Da could not pass into the zygote in about half of the microinjected embryo sacs. This is the first report
of a change in cell-to-cell communication among the cells of the female germ unit before and after fertilization.
Received: 16 December 1999 / Accepted: 4 February 2000 |
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Keywords: | : Female germ unit – Fertilization – Microinjection – Plasmodesmata – Symplastic communication – Torenia (embryo sac) |
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