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Isolation and validation of eight microsatellite loci in a damselfly Ischnura senegalensis (Odonata: Coenagrionidae) by pyrosequencing a bead-enriched library |
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| Authors: | Takaki Kurita Hiroaki Aoyama Seikoh Saitoh Naoya Shinzato Kouji Sawada Takashi Kuriwada Kumiko Hironaka Nobuyuki Inomata Kazunori Yamahira Mamoru Toda |
| Affiliation: | 1. Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan 2. Natural History Museum and Institute, Chiba, 955-2 Aoba-cho, Chuo-ku, Chiba, 260-8682, Japan 3. Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan 4. Fukuoka High School, 1-29-1 Katakasu, Hakata, Fukuoka, 812-0043, Japan 5. Faculty of Education, Kagoshima University, 1-20-6 Korimoto, Kagoshima, 890-0065, Japan 6. Department of Environmental Science, International College of Arts and Sciences, Fukuoka Women’s University, 1-1-1 Kasumigaoka, Higashi-ku, Fukuoka, 813-8529, Japan |
| Abstract: | Eight microsatellite markers for the population genetics and evolutionary ecology of Ischnura senegalensis (Rambur 1842), which shows body color polymorphism in females, were developed using a streptavidin-bead enrichment library and pyrosequence by a next generation sequencer. The number of alleles per locus and effective number of alleles ranged from 3 to 11 and from 1.24 to 5.51, respectively. Observed and expected heterozygosities were 0.18–0.75 and 0.19–0.77, respectively. No linkage disequilibrium between loci was detected. One locus, IsenAC75, deviated significantly from the Hardy–Weinberg equilibrium, and the locus and additional two loci, IsenAC40 and IsenAC8, were suspected for the presence of null alleles. Altogether, these eight microsatellite loci are considered to be useful for population genetic analyses because of the high polymorphic status and independency. |
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