| 毛白杨纤维素合成酶基因(PtoCesA1)克隆、序列分析及其植物表达载体的构建 |
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| 引用本文： | 李春秀,齐力旺,王建华,张守攻.毛白杨纤维素合成酶基因(PtoCesA1)克隆、序列分析及其植物表达载体的构建[J].中国生物工程杂志,2006,26(2):49-53. |
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| 作者姓名： | 李春秀 齐力旺 王建华 张守攻 |
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| 作者单位： | 1. 中国林业科学研究院林业研究所细胞生物学实验室,北京,100091;四川农业大学林学园艺学院,雅安,625014
2. 中国林业科学研究院林业研究所细胞生物学实验室,北京,100091 |
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| 摘 要： | 以毛白杨形成层为材料克隆了毛白杨纤维素合成酶基因（PtoCesA1），序列分析表明该基因序列为3215bp，与欧洲颤杨的PtCesA1基因同源性为97％具有开放的阅读框，编码区在52～2985碱基之间，编码区为2925bp。通过同义突变引入BamHI酶切位点，将全长基因克隆到植物表达栽体pBll21中，经酶切和PCR鉴定确认载体构建正确，为下一步ptoCesA1转基因功能研究打下了基础。
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| 关 键 词： | 毛白杨 纤维素合成酶基因 植物表达载体 |
| 修稿时间： | 2005年5月19日 |
Study on Cellulose Synthase Gene of Chinese White Poplar :Cloning,Sequencing and Construction of Higher Plant Expression Vector |
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| LI Chun-xiu,QI Li-wang,WANG Jian-hua,ZHANG Shou-gong.Study on Cellulose Synthase Gene of Chinese White Poplar :Cloning,Sequencing and Construction of Higher Plant Expression Vector[J].China Biotechnology,2006,26(2):49-53. |
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| Authors: | LI Chun-xiu QI Li-wang WANG Jian-hua ZHANG Shou-gong |
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| Abstract: | The DNA fragment was cloned from the cDNA that was synthesized using the RNA from Chinese white poplar and verified by sequencing This cDNA clone ,designated PtoCesA1, was 3215 bp long with an opening reading frame of 2934bp extending from nucleotides 52~2985.Comprision of the nucleotide sequence of PtoCesA1 with PtrCesA1 showed 97% identity.For construction of the PtoCesA1 binary expression vector, the BamHI site was made by synonymy mutation, the full length PtoCesA1 cDNA was subcloned into pBI121 between the BamHI site and XbaI site. The PtoCesA1 binary vector,containing PtoCesA1,was verified by digestion and PCR. |
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| Keywords: | Chinese white poplar Cellulose synthase gene Higher plant expresstion vector |
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