Tensegrity behaviour of cortical and cytosolic cytoskeletal components in twisted living adherent cells |
| |
Authors: | Laurent Valérie M Cañadas Patrick Fodil Redouane Planus Emmanuelle Asnacios Atef Wendling Sylvie Isabey Daniel |
| |
Affiliation: | (1) INSERM UMR 492, Facultés de Médecine et des Sciences et Technologies, Université Paris XII, Créteil, 94010, France;(2) CNRS UPR 7057-LBHP, Université Paris, VI et VII, PARIS, 75252, France;(3) LBBC-EPFL, PSE-A Ecublens, Lausanne, CH-1015, Switzerland;(4) CNRS UPR 7052-B2OA, Faculté des Sciences et Technologies, Université Paris XII, Créteil, 94010, France;(5) CNRS UPR 7051- LMA-Marseille, 13402, France;(6) INSERM UMR 492, Facultés de Médecine et des Sciences et Technologies, Université Paris XII, Créteil, 94010, France |
| |
Abstract: | The present study is an attempt to relate the multicomponent response of the cytoskeleton (CSK), evaluated in twisted living adherent cells, to the heterogeneity of the cytoskeletal structure - evaluated both experimentally by means of 3D reconstructions, and theoretically considering the predictions given by two tensegrity models composed of (four and six) compressive elements and (respectively 12 and 24) tensile elements. Using magnetic twisting cytometry in which beads are attached to integrin receptors linked to the actin CSK of living adherent epithelial cells, we specifically measured the elastic CSK response at quasi equilibrium state and partitioned this response in terms of cortical and cytosolic contributions with a two-component model (i.e., a series of two Voigt bodies). These two CSK components were found to be prestressed and exhibited a stress-hardening response which both characterize tensegrity behaviour with however significant differences: compared to the cytosolic component, the cortical cytoskeleton appears to be a faster responding component, being a less prestressed and easily deformable structure. The discrepancies in elastic behaviour between the cortical and cytosolic CSK components may be understood on the basis of prestress tensegrity model predictions, given that the length and number of constitutive actin elements are taken into account. |
| |
Keywords: | structural model cytoskeleton actin network magnetic twisting cytometry |
本文献已被 PubMed SpringerLink 等数据库收录! |
|