Microwave-assisted solubilization and solution properties of hyperbranched polysaccharide |
| |
Authors: | Tao Yongzhen Xu Weilin |
| |
Affiliation: | Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan University of Science and Engineering, Wuhan 430073, China |
| |
Abstract: | A water-insoluble hyperbranched β-d-glucan (TM3a), extracted from sclerotia of Pleurotus tuber-regium, was treated by microwave exposure to improve its solubility in water. This method led to complete dissolution of the TM3a polysaccharide in 0.02 wt % aqueous NaN3. Various treatment periods were tested, and optimal conditions corresponded to 35 s at 765 W. The solution properties of TM3a in water were studied systematically by using size-exclusion chromatography combined with laser light scattering, viscometry, and dynamic light scattering at 25 °C. The dependences of intrinsic viscosity ([η]), radius of gyration (), and hydrodynamic radius (Rh) on weight average molecular weight (Mw) for TM3a in 0.02 wt % aqueous NaN3 at 25 °C were found to be , , and in the Mw range from 8.20 × 105 to 4.88 × 106. The fractal dimension, ratio of , and the <r2>o/Mw value of TM3a were calculated and discussed. The results indicated that TM3a existed in a sphere-like conformation in 0.02 wt % aqueous NaN3. Furthermore, by using transmission electron microscopy, we observed directly the spherical molecules of TM3a. This work gave valuable information on improvement of solubility and chain conformation characterization of the water-insoluble polysaccharide in water. |
| |
Keywords: | Hyperbranched polysaccharide Microwave heating Fractal dimension Chain conformation |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|